Penalizing such cases would be overly strict: in the absence of a the gene's genomic context, this looks like a perfectly reasonable alignment (
READ2may fall in a non-coding region and not align, or it may align to another [isolated] coding sequence). As a result, the best way to use paired-end sequencing data with HUMAnN2 is simply to concatenate all reads into a single FASTA or FASTQ file.
catした後に行えば良いみたい。
(2020/4/29: リンク切れしているよう･･･Google groupでの議論にも同様に記載あり)
